GENERAL INSURANCE BLOG

RT-PCR Means Real-Time Polymerase chain reaction. RT-PCR one of the type PCR which the RNA template is first converted into a complementary DNA (cDNA) using reverse transcriptase. The cDNA is then used as a template for exponential amplification using PCR. Enabled sample amplification and eliminated the need for abundant starting material required when using northern blot analysis.

RT-PCR has been used to measure viral load with HIV and may also be used with other RNA viruses such as measles and mumps.

Advantages of real-time PCR test-

1. Amplification and detection occur in a single tube, eliminate post PCR manipulation.

2. An increased dynamic range of detection.

3. Ability to precisely measure the amount of amplicon at each cycle which allows highly accurate quantification of the amount of starting material in samples.

4. Ability to monitor PCR reaction in real-time.

Real-time PCR steps-There are mainly 3 steps involved in real-time PCR Reaction. Reactions generally run for 40 cycles -

1. Denaturation-High temperature incubation is used to melt double standard DNA into single standard DNA and loosen secondary structure in single standard DNA. The highest temperature use for Denaturation is 95 degrees.

2. Annealing-Complementary sequences have an opportunity to hybridize.

3. Extension-At 70-72ºc the activity of the DNA polymerase is optimal, and primer extension occurs at the rates of up to 100 bases per second.

TWO STEP qRT-PCR-Reverse transcriptase PCR (qRT-PCR) starts with the reverse transcription of either RNA or poly (A)+RNA into CDNA.

ONE STEP QRT-PCR-First-strand cDNA synthesis reaction and real-time PCR reaction in the same tube, simplifying reaction setup and reducing the possibility of contamination.

WHAT IS RT-PCR FOR nCOVID-19

The Health Ministry has announced that the real-time reserve transcription-polymerase chain reaction or RT-PCR is the gold standard frontline test for COVID-19.

One is more accurate called RT-PCR which is extracting RNA from the nasal or mouth swab. Because of Genome sequencing technology, we have the sequence of SARS-COV-2 and RNA of the swab is checked whether virus and RNA share the same sequence. Because of proper research and Genome sequence projects going around the world, RT-PCR tests are considered more accurate and reliable.

DISADVANTAGES OF RT-PCR-

RT-PCR Technology is a relatively expensive method. It requires RNA extracting machines, a laboratory and trained technicians. Minimum of 30 samples is needed to make it economically viable.

You’re driving safe. You’ve got your eyes on the road, and your seat belt on. But even if you do everything right, accidents well, they simply happen. While we know you’re doing your best to avoid accidents, here’s a list of essential tips to keep in handy in case you’re in an emergency:

• If you or any of your passengers are injured, medical help is priority. Get first aid immediately.
• Observe the site of the accident and note down all the details you can – from the location, to important number plates and names.
• If there are eye-witnesses to the accident, make sure you note down their contact details, so you can follow up with them later.
• Take pictures of the place where the accident happened. In times like these, every detail helps!
• If the police have registered a case, remember to note down the case number.
• Keep track of all the expenses you’ve had to undergo due to the accident. That means, don’t throw a single bill or receipt away!
• Lastly, make sure you file your insurance claim as soon as you can! Because the sooner your Insurance Company knows about the incident, the sooner they can help you out.

In case of an unfortunate incident on the road, our no. 1 tip for you is the simplest: don’t panic! Stay calm, and keep a level head.
No matter where you are, always keep the accident no. on speed dial
Here’s to many safe, stress-free road-trips!

Started from the province of Wuhan in China, COVID-19 has spread all around the world, leaving behind the massacres. First reported on 30th January 2020, India has recorded a total of 1.44 million active cases and more than 32 thousand people have lost their lives as reported till 27th July 2020. Yet, till the current date, no verified vaccines have been launched in the market leaving behind a massive matter of concern for the common masses. In incurring medical care, what comes up to our mind first, does health insurance in India cover the COVID-19 or not?

What expenses does the health insurance cover?

Current Coronavirus health insurance in India covers hospitalization expenses including in-patient hospitalization, pre-hospitalization and post-hospitalization expenses. But it's always better to get our immunity system to act as the main barrier to trust, and for that, we need a stone-hard immunity.

What do you need for better immunity?

To get better immunity, we must focus on our nutritional well being and good food habits. To boost your immune system, you need the essential macro and micronutrients like proteins, good fats, carbohydrates, inorganic elements, and essential vitamins, apart from looking at the health insurance in India.

Some foods that you must include in your diet

1. Milk: One of the most common and cheap nutrition-rich liquid food is milk. A cup of dairy milk contains almost 8 grams of protein, 12 grams of carbohydrates, and 2.4 grams of fats along with Vitamin A, Vitamin D, and Vitamin B12. It is also a good source of Calcium(Ca) and Potassium(K). Due to the high complete protein content, it helps in lean muscle building and keeps bones healthy too. Having one cup of milk a day could help us keep us on track with the daily macros and micro required to boost up our immunity.

2. Egg: A whole egg contains approximately 13 grams of protein, 1.1 gram of carbohydrates, 11g of good fats along with Vitamin A, VItamin B2, B5, B6, B12, Vitamin D, Vitamin E, and Vitamin K. The high-quality proteins and Essential Amino Acids in egg make it a complete source of protein. It is also a great source of Calcium, Zinc, and Iron. It raises HDL, which reduces the risks of heart disease.

3. Chicken: With a protein content of 27g, good fats of 14 grams, chicken is one of the best low-fat protein sources. It maintains muscle mass, strengthens bones, and reduces appetite. Though fried chickens might not be too good for our health, preferably grilled or boiled chicken, should be consumed for better outcomes.

4. Fruits: A variety of foods must be consumed regularly. Different fruits have different useful nutrient facts.
I. Banana: It has a high source of good carbohydrates. It also contains the right amount of Iron, Potassium, and Vitamin C. It is also a good source of fibres that keep our intestine healthy.
II. Lemons: The most famous citrus fruit, Lemons are highly rich in Vitamin C, Potassium, and fibres. Vitamin C helps in reducing heart disease risk, and the citric acid in lemons reduces kidney stones.
III. Grapes: Grapes packed with nutrients, especially Vitamin C and K are an excellent antioxidant that may prevent chronic diseases. It may reduce cholesterol and also might decrease blood sugar levels.

A balanced and well nutritious diet can help you increase your immunity to combat COVID-19 effectively. With healthy food, a bit of proper exercise will enhance your metabolism. Different health insurance in India promotes self-awareness so that the policyholders stay away from medical help.

It’s time we shorten the time to Claim.

You know what’s the worst part about making a claim? THE WAITING! That uncertain period of time between you submitting your case, and your insurance company responding with a simple ‘Yes’ or ‘No’. We have all been through the ordeal, and we have all resigned to the bitter fact that there’s nothing you can do to change the situation.
Well, we decided we could do something about it! And we did!
Here’s how we manage to deliver seamless claims.

No waiting

You don’t have to wait for a third-party inspection, which may take days. Just do a quick self-survey using your smartphone camera by clicking pictures of the damage and send it to us. That’s it, your inspection is done in minutes!

No hard-copies

We don’t ask you for hard copies. We live true to our name and make everything ‘digital’. Which means, for any documentation or proof, just send us a soft-copy over email.

Our Customer Care works 24x7, making sure your claims are approved faster